Open AccessCell cycle regulation ofcdc25Ctranscription is mediated by the periodic repression of the glutamine-rich activators NF-Y and Sp1
Author(s) -
Jörk Zwicker,
Claudia Groß,
Frances C. Lucibello,
Mathias Truss,
F Ehlert,
Kurt Engeland,
Rolf Müller
Publication year - 1995
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/23.19.3822
Subject(s) - biology , psychological repression , transcription (linguistics) , repressor , transcription factor , upstream activating sequence , microbiology and biotechnology , dna binding protein , binding site , gene , genetics , gene expression , linguistics , philosophy , enhancer
The late S/G2-specific transcription of the human cdc25C gene is dependent on an initiator-proximal repressor element (CDE) and an upstream activating sequence (UAS) of undefined nature. We now show that these upstream sequences harbour multiple in vivo protein binding sites that interact with transcriptional activators and form separable, context-independent functional modules. Major components of the UAS are a bona fide Sp1 site and three direct sequence repeats (Yc-boxes). The Yc-boxes interact with the CCAAT-box binding protein NF-Y and are critically dependent on synergistic interactions for efficient transcription activation. The NF-Y complexes, as well as Sp1, are constitutive activators, whose activation function is periodically repressed through the CDE. These observations indicate that the cell cycle regulation of cdc25C transcription is mainly due to the CDE-mediated repression of glutamine-rich activators.