Open AccessThe actinophage RP3 DNA integrates site-specifically into the putative tRNAArg(AGG) gene ofStreptomyces rimosus
Author(s) -
Gabriel Karin,
Holger Schmid,
Ute Schmidt,
Helmut Rausch
Publication year - 1995
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/23.1.58
Subject(s) - biology , dna , transfer rna , gene , genetics , streptomycetaceae , streptomyces , microbiology and biotechnology , biochemistry , actinomycetales , rna , bacteria
The temperate actinophage RP3 integrates site-specifically into the chromosome of Streptomyces rimosus R6-554. The phage attachment site attP and the hybrid attachment sites of the integrated prophage--attL and attR--were cloned and sequenced. The 54nt core sequence, common to all RP3 related attachment sites, comprises the 3' terminal end of a putative tRNA(Arg)(AGG) gene. AttB bears the complete tRNA gene which is restored in attL after integration. A 7.5kb HindIII fragment, bearing attP, was used to construct an integrative plasmid to simulate the integration process in vivo and to localize the phage genes necessary for site specific integration. The int and xis genes were sequenced and compared to other recombination genes.