Open AccessIntramolecular recombination in polymavirus DNA is controlled by promoter elements
Author(s) -
Chantal Nault,
Stéphane Veilleux,
Louis Delbecchi,
Danielle Bourgaux-Ramoisy,
Pierre Bourgaux
Publication year - 1994
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/22.3.485
Subject(s) - biology , recombination , homologous recombination , transcription (linguistics) , dna , promoter , genetics , flp frt recombination , dna replication , v(d)j recombination , intramolecular force , viral replication , microbiology and biotechnology , genetic recombination , gene , virus , physics , linguistics , philosophy , gene expression , quantum mechanics
We show here that intramolecular homologous recombination in polyomavirus (Py) DNA depends upon discrete sequence elements of the viral regulatory region which are believed to regulate transcription initiation and exert little or no cis-control over replication. Either deleting the viral early promoter (EP) or inverting the viral late promoter (LP) strongly impairs viral DNA recombination under conditions allowing viral DNA replication to proceed undisturbed. These findings suggest that bi-directional transcription proceeding from the intergenic region favors intramolecular recombination.