RAP1 stimulates single- to double-strand association of yeast telomeric DNA: implications for telomere - telomere interactions | Zendy

Eric Gilson | Zendy; Thomas Mulled | Zendy; José M. Sogo | Zendy; Thierry Laroche | Zendy; Susan M. Gasser | Zendy

Open Access

RAP1 stimulates single- to double-strand association of yeast telomeric DNA: implications for telomere - telomere interactions

Author(s) -

Eric Gilson,

Thomas Mulled,

José M. Sogo,

Thierry Laroche,

Susan M. Gasser

Publication year - 1994

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/22.24.5310

Subject(s) - biology , telomere , telomere binding protein , rap1 , dna , homologous recombination , genetics , gene , microbiology and biotechnology , dna binding protein , transcription factor

Repressor Activator Protein 1 (RAP1) of Saccharomyces cerevisiae is an abundant nuclear protein implicated in telomere length maintenance, transactivation, and in the establishment of silent chromatin domains. The RAP1 binding site 5' of the yeast HIS4 gene is also a region of hyperrecombination in meiosis. We report here that as RAP1 binds its recognition consensus, it appears to untwist double-stranded DNA, which we detect as the introduction of a negative supercoil in circularization assays. Coincident with the RAP1-dependent untwisting, we observe stimulation of the association of a single-stranded yeast telomeric sequence with its homologous double-stranded sequence in a supercoiled plasmid. This unusual distortion of the DNA double helix by RAP1 may contribute to the RAP1-dependent enhancement of recombination rates and promote non-duplex strand interactions at telomeres.

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