Re-replication of SV40 minichromosomes is inhibited at the stage of chain elongation | Zendy

Torsten Krude | Zendy; Anja Winter | Zendy; Rolf Knippers | Zendy

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Re-replication of SV40 minichromosomes is inhibited at the stage of chain elongation

Author(s) -

Torsten Krude,

Anja Winter,

Rolf Knippers

Publication year - 1994

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/22.24.5265

Subject(s) - biology , elongation , replication (statistics) , genetics , dna replication , microbiology and biotechnology , minichromosome , chain (unit) , dna , computational biology , virology , chromatin , materials science , ultimate tensile strength , metallurgy , physics , astronomy

The template activities of protein-free SV40 DNA and SV40 minichromosomes for DNA re-replication are compared in in vitro replication assays. Density substitution experiments and two-dimensional gel electrophoresis show that protein-free DNA can replicate for at least two cycles whereas salt-treated minichromosomes replicate only once. Re-replication of minichromosomes is blocked at the stage of replicative chain elongation suggesting that replicatively assembled chromatin has structural features that prevent a second round of replication.

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