Open AccessModified RNA sequence pools forin vitroselection
Author(s) -
Yun Lin,
Qiu Qiu,
Stanley C. Gill,
Sumedha D. Jayasena
Publication year - 1994
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/22.24.5229
Subject(s) - biology , rna , rnase p , in vitro , nuclease protection assay , rnase h , elastase , biochemistry , cleavage (geology) , microbiology and biotechnology , non coding rna , gene , enzyme , paleontology , fracture (geology)
We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.