Modified RNA sequence pools forin vitroselection | Zendy

Yun Lin | Zendy; Qiu Qiu | Zendy; Stanley C. Gill | Zendy; Sumedha D. Jayasena | Zendy

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Modified RNA sequence pools forin vitroselection

Author(s) -

Yun Lin,

Qiu Qiu,

Stanley C. Gill,

Sumedha D. Jayasena

Publication year - 1994

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/22.24.5229

Subject(s) - biology , rna , rnase p , in vitro , nuclease protection assay , rnase h , elastase , biochemistry , cleavage (geology) , microbiology and biotechnology , non coding rna , gene , enzyme , paleontology , fracture (geology)

We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.

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