Open AccessPreparation of a DNA matrix via an electrqchemically directed copolymerization of pyrrole and oligonucleotides bearing a pyrrole group
Author(s) -
Thierry Livache,
André Roget,
Emmanuel Dejean,
Christelle Barthet,
Gérard Bidan,
R. Téoule
Publication year - 1994
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/22.15.2915
Subject(s) - pyrrole , oligonucleotide , copolymer , polypyrrole , phosphoramidite , polymer , moiety , dna , combinatorial chemistry , materials science , covalent bond , polymer chemistry , electrode , polymerization , biology , organic chemistry , chemistry , biochemistry , composite material
A new methodology for the preparation of addressed DNA matrices is described. The process includes an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing on their 5' end a pyrrole moiety introduced by phosphoramidite chemistry. The electro-controlled synthesis of the copolymer (poly-pyrrole) gives, in one step, a solid conducting film deposited on the surface of an electrode. The resulting polymer consists of pyrrole chains bearing covalently linked oligonucleotide. The polymer growth is limited to the electrode surface, so that it is possible to prepare a DNA matrix on a multiple electrode device by successive copolymerizations. A support bearing four oligonucleotides was used to detect three ras mutations on a synthetic DNA fragment.