Open AccessUse of a transactive regulatory mutant ofDictyostelium discoideumin a eucaryotic expression system
Author(s) -
Birgit Wetterauer,
Klaus Salger,
Harry K. MacWilliams
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.6.1397
Subject(s) - dictyostelium discoideum , biology , heterologous , dictyostelium , mutant , luciferase , heterologous expression , gene , mutation , promoter , microbiology and biotechnology , reporter gene , regulation of gene expression , repressor , gene expression , genetics , transfection , recombinant dna
The discoidin proteins of Dictyostelium discoideum are highly expressed during development. The Disc I gamma promoter allows the regulation of heterologous protein expression by experimental conditions. We report conditions under which the promoter activity is efficiently repressed during growth in the wildtype strain AX2. In addition we show that a mutant which overexpresses the discoidins also overexpresses the reporter genes beta-galactosidase, luciferase and CAT 10- to 100-fold when these are placed under the control of a Disc I gamma promoter. This system may be generally useful for the overexpression of genes in Dictyostelium, both for functional studies in vivo and for the production of heterologous proteins for purification.