Open AccessBI- BIItransitions in B-DNA
Author(s) -
Brigitte Hartmann,
D. Piazzola,
Richard Lavery
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.3.561
Subject(s) - dodecameric protein , biology , dna , crystallography , cpg site , transition (genetics) , sequence (biology) , nuclear magnetic resonance spectroscopy , biophysics , stereochemistry , genetics , gene , chemistry , gene expression , dna methylation
Molecular modelling is used to study the conformational and energetic aspects of BI-BII transitions within the backbone of a B-DNA dodecamer d(CATGACGTCATG) whose fine structure has previously been determined by molecular modelling combined with NMR spectroscopy. It is shown that while the dodecamer under investigation does not contain any BII junctions, the central CpG step can most easily undergo the transition. More generally, it is also found that the base sequence and hence the backbone geometry of a DNA segment, strongly influences both the conformational impact of the transition, the associated energy barrier and the stability of the resulting BII state.