Distinct DNA binding preferences for the c-Myc/Max and Max/Max dimers | Zendy

David L.C. Solomon | Zendy; Bruno Amati | Zendy; Hartmut Land | Zendy

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Distinct DNA binding preferences for the c-Myc/Max and Max/Max dimers

Author(s) -

David L.C. Solomon,

Bruno Amati,

Hartmut Land

Publication year - 1993

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/21.23.5372

Subject(s) - transactivation , biology , leucine zipper , dimer , transcription factor , dna , dna binding protein , genetics , binding site , base pair , gene , transcription (linguistics) , basic helix loop helix leucine zipper transcription factors , microbiology and biotechnology , physics , linguistics , philosophy , nuclear magnetic resonance

The transcription factor c-Myc and its dimerisation partner Max are members of the basic/helix-loop-helix/leucine-zipper (bHLH-Z) family and bind to the DNA core sequence CACGTG. Using a site-selection protocol, we determined the complete 12 base pair consensus binding sites of c-Myc/Max (RACCACGTGGTY) and Max/Max (RANCACGTGNTY) dimers. We find that the c-Myc/Max dimer fails to bind the core when it is flanked by a 5'T or a 3'A, while the Max/Max dimer readily binds such sequences. Furthermore we show that inappropriate flanking sequences preclude transactivation by c-Myc in vivo. In conclusion, Max/Max dimers are less discriminatory than c-Myc/Max and may regulate other genes in addition to c-Myc/Max targets.

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