Open Access3' truncated tRNAArgis essential forin vitrospecific cleavage of partially synthesized mouse 18S rRNA
Author(s) -
Masayuki Nashimoto
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.20.4696
Subject(s) - rna , biology , transfer rna , ribonuclease , microbiology and biotechnology , biochemistry , in vitro , cleavage (geology) , nuclease , nucleotide , nuclease protection assay , micrococcal nuclease , ribosomal rna , dna , non coding rna , gene , paleontology , nucleosome , fracture (geology) , histone
In vitro synthesized 5' portions of mouse 18S rRNA are cleaved efficiently at a specific site in partially purified extracts of mouse FM3A cells and several mouse tissues. This activity is composed of both protein and RNA, and can be reconstituted with the protein component in micrococcal nuclease-treated extracts and the RNA component in phenol-treated ones. The RNA component of about 65 nucleotides with the complementing activity was purified from total RNA in the partially purified FM3A cell extracts by polyacrylamide gel electrophoreses. Chemical sequencing of this RNA elucidated that it is tRNAArg lacking nine nucleotides from its 3' terminus. Ribonuclease H treatment directed by deoxyoligonucleotides complementary to tRNAArg completely abolishes the cleavage activity, supporting the above conclusion.