Open AccessStudies on the synthesis of nucleotidyl-peptides. II. The preparation of a nucleoidyl-peptide haVING A 5'-nucleotidyl-(p-o)-serine phosphodiester bond
Author(s) -
Yoshihito Ueno,
Ryuichi Saito,
Tsujiaki Hata
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.19.4451
Subject(s) - phosphodiester bond , biology , serine , peptide , biochemistry , microbiology and biotechnology , phosphorylation , stereochemistry , rna , gene , chemistry
The preparation of a nucleotidyl-peptide having a thymidine-5'-yl-(P-O)-serine phosphodiester bond, [H-Ala-Ser(pTpT)-Phe-OH](24) is described. After condensation between the phosphorylated peptide component and an oligonucleotide component, all protecting groups could be removed under neutral conditions without beta-elimination of the pTpT from the serine moiety.