Open AccessDisruption in the AU motif of the mouse TNF-α3′ UTR correlates with reduced TNF production by macrophagesin vitro
Author(s) -
Chaim O. Jacob,
Nancy Beerman Tashman
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.11.2761
Subject(s) - biology , untranslated region , gene , sequence motif , three prime untranslated region , microbiology and biotechnology , messenger rna , tumor necrosis factor alpha , genetics , cytokine , base pair , in vitro , gene expression , immunology
Many cytokine mRNAs exhibit a conserved, AU-rich motif in the 3'-untranslated region (UTR) of the molecule. Such sequence elements have been implicated in the regulation of mRNA turnover and as potential translational regulators. We report on the identification of a 3 base pair insertion which disrupts the AU motif of the TNF-alpha gene in the NZW, B10.KPA44, SM/J and Mus spretus mice and an insertion of an 8 base pair sequence into the 3' AU motif of the IL-10 gene in the Mus Spretus mouse. The mutation in the AU motif of the TNF-alpha gene correlates with reduced production of this cytokine by peritoneal macrophages from these mouse strains.