Open AccessSelective binding of the estrogen receptor to one strand of the estrogen responsive element
Author(s) -
Ranjan Mukherjee
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.11.2655
Subject(s) - biology , hormone response element , palindromic sequence , xenopus , microbiology and biotechnology , coding strand , oligonucleotide , binding site , estrogen receptor , transcription (linguistics) , estrogen , gene , nucleotide , palindrome , biochemistry , genetics , linguistics , philosophy , cancer , breast cancer , crispr
The human estrogen receptor (hER) activates gene transcription by binding to cognate palindromic sequences called estrogen responsive elements (ERE). I used gel retardation assays and oligonucleotides containing the ERE from the Xenopus vitellogenin gene to study the interaction of the hER with the ERE. I observed that the hER bound to double-stranded ERE and to the single strand of the ERE that had T in the center with nearly equal affinity, but not to the strand which had A in the center. Interchanging the two central nucleotides changed the strand specificity. Binding of the hER to a single strand is extremely sensitive to temperature. Initial recognition of one of the two strands of the ERE may be involved in the binding of the hER to the ERE.