Open AccessCell type-specific interactions of transcription factors with a housekeeping promoterin vivo
Author(s) -
Genevieve Stapleton,
Maria Patrizia Somma,
Patrizia Lavia
Publication year - 1993
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/21.10.2465
Subject(s) - biology , housekeeping gene , promoter , footprinting , gene , transcription factor , housekeeping , genetics , tata box , dna footprinting , transcription (linguistics) , dna , regulatory sequence , computational biology , gene expression , linguistics , philosophy
Mammalian housekeeping promoters represent a class of regulatory elements different from those of tissues-specific genes, lacking a TATA box and associated with CG-rich DNA. We have compared the organization of the housekeeping Htf9 promoter in different cell types by genomic footprinting. The sites of in vivo occupancy clearly reflected local combinations of tissue-specific and ubiquitous binding factors. The flexibility of the Htf9 promoter in acting as the target of cell-specific combinations of factors may ensure ubiquitous expression of the Htf9-associated genes.