Open AccessMutations inE.Coli16S rRNA that enhance and decrease the activity of a Suppressor tRNA
Author(s) -
Catherine Prescott,
Hans–Christian Kornau
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.7.1567
Subject(s) - transfer rna , biology , suppressor , nonsense mutation , genetics , ribosomal rna , mutation , nonsense , suppressor mutation , microbiology and biotechnology , gene , rna , missense mutation
The in vivo expression of mutations constructed within helix 34 of 16S rRNA has been examined together with a nonsense tRNA suppressor for their action at stop codons. The data revealed two novel results: in contrast to previous findings, some of the rRNA mutations affected suppression at UAA and UAG nonsense codons. Secondly, both an increase and a decrease in the efficiency of the suppressor tRNA were induced by the mutations. This is the first report that rRNA mutations decreased the efficiency of a suppressor tRNA. The data are interpreted as there being competition between the two release factors (RF-1 and RF-2) for an overlapping domain and that helix 34 influences this interaction.