Open AccessA physical and genetic map of theSpiroplasma citrigenome
Author(s) -
Fengchun Ye,
Frédéric Laigret,
Jane C. Whitley,
Christine Citti,
Lloyd R. Finch,
Patricia Carle,
Joël Renaudin,
J.M. Bové
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.7.1559
Subject(s) - spiroplasma , mollicutes , biology , genome , genetics , mycoplasmataceae , genome size , mycoplasma mycoides , restriction map , mycoplasma , restriction enzyme , gene , nucleic acid sequence
A physical and genetic map of the Spiroplasma citri genome has been constructed using several restriction enzymes and pulsed field gel electrophoresis. A number of genes were subsequently localized on the map by the use of appropriate probes. The genome size of the spiroplasma estimated from restriction fragments is close to 1780 kbp, the largest of all Mollicutes studied so far. It contains multisite insertions of Spiroplasma virus 1 (SpV1) sequences. The physical and genetic map of the S. citri genome shares several features with that of other Mollicutes, especially those in the Mycoplasma mycoides cluster. This supports the finding that S. citri and these Mycoplasma spp. are phylogenetically related.