Open Access3′ Alu PCR: a simple and rapid method to isolate human polymorphic markers
Author(s) -
Jean-Paul Charlieu,
André Laurent,
D A Carter,
Michel Bellis,
Gérard Roizès
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.6.1333
Subject(s) - biology , alu element , genetics , polymerase chain reaction , simple (philosophy) , genetic marker , computational biology , gene , human genome , genome , philosophy , epistemology
Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the microsatellites sequences in order to design specific PCR primers before testing for polymorphism, which does not always occur. We propose here a new approach to generate polymorphic markers, based on the amplification of microsatellites at the 3' end of Alu sequences, without the need for cloning or sequencing steps.