Processing in the 5′ region of the pnp transcript facilitates the site-specific endonucleolytic cleavages of mRNA | Zendy

Renkichi Takata | Zendy; Mika Izuhara | Zendy; Koichi Akiyama | Zendy

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Processing in the 5′ region of the pnp transcript facilitates the site-specific endonucleolytic cleavages of mRNA

Author(s) -

Renkichi Takata,

Mika Izuhara,

Koichi Akiyama

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.4.847

Subject(s) - biology , polynucleotide phosphorylase , rnase p , cleavage (geology) , messenger rna , gene , microbiology and biotechnology , primary transcript , ribonuclease iii , rnase h , ribonuclease , escherichia coli , genetics , rna , enzyme , biochemistry , purine nucleoside phosphorylase , rna interference , alternative splicing , paleontology , purine , fracture (geology)

The primary transcript of pnp, the gene encoding polynucleotide phosphorylase in Escherichia coli, is processed in the 5' end region by ribonuclease III (RNase III). The unprocessed transcript shows enhanced stability compared with the processed transcript. We report here that, unlike the processed transcript, the unprocessed pnp transcript did not accept endonucleolytic attack at, at least, five cleavage sites. Sequencing analysis of the four cleavage products shows no sequence specific to all these sites, but AU rich stretches were observed at three sites.

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