Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco | Zendy

Danuta Chamot | Zendy; Cris Kuhlemeier | Zendy

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Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco

Author(s) -

Danuta Chamot,

Cris Kuhlemeier

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.4.665

Subject(s) - biology , complementary dna , polyclonal antibodies , gene , microbiology and biotechnology , amino acid , coding region , peptide sequence , antiserum , translation (biology) , genetics , nicotiana tabacum , messenger rna , antibody

Two Nicotiana plumbaginifolia cDNA clones, NeIF-5A1 and NeIF-5A2, encoding eukaryotic translation initiation factor eIF-5A (formerly called eIF-4D) were cloned by heterologous screening with Dictyostelium and human eIF-5A probes. eIF-5A is the only protein known to contain a unique amino acid modification, hypusine. Comparison of the Nicotiana deduced amino acid sequences with those of other eIF-5A polypeptides reveals conservation throughout the coding sequence, especially in the region of the hypusine residue. Transcript analysis reveals that NeIF-5A1 is preferentially expressed in photosynthetic tissues, while NeIF-5A2 is constitutively expressed in all plant tissues examined. A polyclonal antibody was raised against NeIF-5A1 overexpressed in E. coli. NeIF-5A1 antiserum crossreacts with an 18 kDa polypeptide doublet in all tobacco tissues examined. At least one polypeptide of ca. 18 kDa from a diversity of higher and lower plants crossreacts with NeIF-5A1 antiserum.

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