Open AccessStoichiometry of the Cre recombinase bound to theloxrecombining site
Author(s) -
Alison Mack,
Brian Sauer,
Kenneth Abremski,
Ronald H. Hoess
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.17.4451
Subject(s) - biology , recombinase , site specific recombination , recombination , binding site , palindrome , cre recombinase , bacteriophage , inverted repeat , palindromic sequence , footprinting , a site , dna footprinting , microbiology and biotechnology , genetics , molecule , biophysics , dna , base sequence , dna binding protein , physics , transcription factor , gene , escherichia coli , transgene , genome , quantum mechanics , genetically modified mouse
The site-specific recombinase Cre from bacteriophage P1 binds and carries out recombination at a 34 bp lox site. The lox site consists of two 13 bp inverted repeats, separated by an 8 bp spacer region. Both the palindromic nature of the site and the results of footprinting and band shift experiments suggest that a minimum of two Cre molecules bind to a lox site. We report here experiments that demonstrate the absolute stoichiometry of the Cre-lox complex to be one molecule of Cre bound per inverted repeat, or two molecules per lox site.