Open AccessCloning of the gene for the 73kD subunit of the DNA polymerase α primase of Drosophila Melanogaster
Author(s) -
Sue Cotterill,
Lehman Ir,
P McLachlan
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.16.4325
Subject(s) - biology , primase , drosophila melanogaster , cloning (programming) , genetics , gene , dna polymerase , molecular cloning , primer (cosmetics) , polymerase , protein subunit , microbiology and biotechnology , polymerase chain reaction , complementary dna , reverse transcriptase , chemistry , organic chemistry , computer science , programming language
We have isolated both cDNA and genomic clones for the 73 kDa subunit of the DNA polymerase alpha primase of Drosophila melanogaster. Analysis of these clones has identified an open reading frame of 1959 bases coding for a protein of 72.5 kDa. Northern analysis has shown the mRNA for the gene to be approximately 2.5 kb, and comparison of the cDNA and the genomic clones shows that the coding region of the gene lacks introns. The 5' end of the transcript has been mapped by primer extension, and the position of the gene in the genome mapped using in situ analysis. Computer analysis has been carried out on both coding and non coding regions of the gene. The protein sequence shows some homology to the analogous subunit in the S. cerevisiae DNA polymerase alpha, however a search of the data banks failed to reveal other homologies, or provide any clues as to the function of the protein. Analysis of the non-coding regions indicates some potential control regions for the gene. The 73 kDa protein has been overproduced, but a preliminary analysis failed to reveal any enzymatic activities.