Open AccessSolid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAGTAAGCC]-Glu-OH from the nucleoprotein ofBacillus Subtilisphage ø29
Author(s) -
C.M. Dreef-Tromp,
J.C.M. van der Maarel,
Hans van den Elst,
Gijs A. van der Marel,
Jacques H. van Boom
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.15.4015
Subject(s) - biology , bacillus subtilis , oligonucleotide , nucleoprotein , dna , bacteriophage , fragment (logic) , solid phase synthesis , stereochemistry , biochemistry , microbiology and biotechnology , combinatorial chemistry , escherichia coli , bacteria , genetics , gene , chemistry , peptide , computer science , programming language
The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using N-2-(tert-butyldiphenylsilyloxymethyl)benzoyl protected nucleosides. The oligonucleotide was linked via the extremely base-labile oxalyl ester anchor to the solid support.