Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAGTAAGCC]-Glu-OH from the nucleoprotein ofBacillus Subtilisphage ø29 | Zendy

C.M. Dreef-Tromp | Zendy; J.C.M. van der Maarel | Zendy; Hans van den Elst | Zendy; G. A. VAN DER MAREL | Zendy; Jacques H. van Boom | Zendy

Open Access

Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAGTAAGCC]-Glu-OH from the nucleoprotein ofBacillus Subtilisphage ø29

Author(s) -

C.M. Dreef-Tromp,

J.C.M. van der Maarel,

Hans van den Elst,

G. A. VAN DER MAREL,

Jacques H. van Boom

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.15.4015

Subject(s) - biology , bacillus subtilis , oligonucleotide , nucleoprotein , dna , bacteriophage , fragment (logic) , solid phase synthesis , stereochemistry , biochemistry , microbiology and biotechnology , combinatorial chemistry , escherichia coli , bacteria , genetics , gene , chemistry , peptide , computer science , programming language

The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using N-2-(tert-butyldiphenylsilyloxymethyl)benzoyl protected nucleosides. The oligonucleotide was linked via the extremely base-labile oxalyl ester anchor to the solid support.

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