Open AccessSplice site selection by intron a13 of the COX1 gene fromSaccharomyces cerevisiae
Author(s) -
Arend J. Winter,
Marian J. A. Groot Koerkamp,
Henk F. Tabak
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.15.3897
Subject(s) - intron , splice site mutation , splice , biology , rna splicing , exon , genetics , gene , group i catalytic intron , nucleotide , alternative splicing , rna
Interactions of the 5' and 3' splice sites with intron internal sequences of the yeast mitochondrial group I intron aI3 were studied using mutation analysis. The results can be fully explained by the splice guide model in which the splice sites are defined by the Internal Guide Sequence. No evidence was found for an alternative interaction between intron nucleotides preceding the 3' splice site and nucleotides in the vicinity of the core region as was found for the Tetrahymena intron. Our results also suggest that binding of the 5' and 3' splice site nucleotides to the IGS can not take place simultaneously. The intron must therefore undergo conformational changes as the reaction proceeds from the first step of self splicing, GTP attack at the 5' splice site, to exon ligation, the second step.