Two promoter elements are necessary and sufficient for expression of the sea urchin UI snRNA gene | Zendy

Brian J. Wendelburg | Zendy; William F. Marzluff | Zendy

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Two promoter elements are necessary and sufficient for expression of the sea urchin UI snRNA gene

Author(s) -

Brian J. Wendelburg,

William F. Marzluff

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.14.3743

Subject(s) - biology , sea urchin , small nuclear rna , snrnp , gene , transcription (linguistics) , genetics , microbiology and biotechnology , gene expression , rna splicing , rna , linguistics , philosophy , non coding rna

The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between -318 and -300 and a proximal sequence element (PSE) centered at -55. Removal or alteration of other sequences conserved in different sea urchin snRNA U1 genes, including deletion of all sequence between -90 and -273, did not affect the expression. Sequences around the start site were not required for expression. Deletion of nucleotides between the PSE and the start site resulted in initiation inside the U1 coding region, suggesting that the PSE determines the start site of transcription. There is no obvious similarity between the sequences required for the sea urchin U1 snRNA expression and the sequences required for the expression of other sea urchin snRNAs.

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