Open AccessHeterologous activation of the actinorhodin biosynthetic pathway inStreptomyces lividans
Author(s) -
Natália Romero,
Vı́ctor Parro,
Francisco Malpartida,
Rafael P. Mellado
Publication year - 1992
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/20.11.2767
Subject(s) - actinorhodin , biology , heterologous , library science , genetics , streptomyces , streptomyces coelicolor , gene , computer science , bacteria
A DNA fragment of Streptomyces fradiae is able to activate the antibiotic actinorhodin biosynthetic pathway when cloned in Streptomyces lividans. The activator DNA region has been sequenced and its transcription initiation and termination sites accurately mapped in vivo. This DNA encodes a 132 nucleotides long transcript which is apparently responsible for the actinorhodin production phenotype, possibly acting as an antisense RNA. The sequence of the activator gene revealed no homology with any other known Streptomyces coelicolor genes concerned with actinorhodin biosynthesis or its pleiotropic regulation.