Open AccessSingle strand comformation of adenylate chains analysed by a specific photoreaction. Determination of structure by 5′ residue
Author(s) -
Dietmar Pörschke
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.9.1621
Subject(s) - photodegradation , residue (chemistry) , quantum yield , adenylate kinase , nucleotide , biology , yield (engineering) , stereochemistry , photochemistry , materials science , chemistry , biochemistry , enzyme , photocatalysis , fluorescence , catalysis , physics , quantum mechanics , gene , metallurgy
The photoreactivity was analysed in various oligo- and polyadenylates: 1) The quantum yields of the specific photoreaction in poly(dA) and dApdA decrease with increasing temperature, whereas the quantum yield of the photodegradation in poly(rA) increases. 2) The photoreactivities of poly(2'MeA) and poly(2'EtA) closely correspond to that of poly(rA). 3) The photodegradation of rApdA is very similar to that of rAprA, whereas dAprA shows the same specific photoreaction as observed for dApdA. These data support the view, that the specific photoreaction observed in oligo(dA) and poly(dA) is dependent upon a specific conformation, which is not accessible to oligo(rA), poly(rA), poly(2'MeA) and poly(2'EtA). The specific conformation is determined by the nucleotide, which carries the internucleotide bond in the 3'-position.