Open Accessm7G5′ppp5′GmpCpUp at the 5′ terminus of reovirus messenger RNA
Author(s) -
Manny Faust,
Kenneth E.M. Hastings,
S. Milward
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.8.1329
Subject(s) - guanosine , biology , messenger rna , rna , microbiology and biotechnology , nucleotide , polyadenylation , methionine , guanosine triphosphate , biochemistry , amino acid , gene
In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' terminal sequence of methylated reovirus mRNA revealed that it has the structure m7G5'ppp5'GmpCpUp.