Open AccessThe cleavage of transfer RNA by a single strand specific endonuclease from Neurospora crassa
Author(s) -
Jacov Tal
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.7.1073
Subject(s) - neurospora crassa , transfer rna , biology , endonuclease , neurospora , polynucleotide , cleavage (geology) , crassa , cleave , biochemistry , rna , enzyme , nucleotide , genetics , microbiology and biotechnology , gene , paleontology , fracture (geology) , mutant
Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaved in the anticodon region. The 3' terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.