Open AccessIsolation of endonuclease from mammalian cells by DNA - cellulose chromatograpby
Author(s) -
Annalisa Caputo,
Gabriella Zupi,
Anna Clanciulli
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.6.905
Subject(s) - biology , endonuclease , dna , isolation (microbiology) , genetics , cellulose , microbiology and biotechnology , biochemistry
By means of DNA-cellulose chromatography an enzyme with endonucleolytic activity has been isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, eluted at 0.7 M NaCl, effects the velocity sedimentation of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely inactive using native as well as heat-denatured DNA.