Open AccessPoly(U, s4U) as a synthetic messenger RNA for polyphenylalanine synthesis in a cell-free system derived from rat liver
Author(s) -
M. Keren-Zur,
Abraham Hochberg,
Nathan de Groot,
Y. Lapidot
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.4.595
Subject(s) - biology , messenger rna , cell free system , rna , transfer rna , phenylalanine , biochemistry , protein biosynthesis , amino acid , microbiology and biotechnology , enzyme , gene
Two different U and S4-U containing polymers with U:s4U ratio of 3:1 and 5:1 were synthesized. Their activity as messenger RNA was studied in an amino acid incorporation cell-free system from rat liver. It was shown that both copolymers can stimulate the incorporation of phenylalanine into oligophynylalamyl-tRNA.