Open AccessConstruction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide2-14of bovine ribonuclease A
Author(s) -
Clifford L. Harvey,
Kenneth Olson,
Alice De Czekala,
A. L. Nussbaum
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.11.2007
Subject(s) - biology , deoxyribonucleotide , ribonuclease , duplex (building) , dna , base pair , sequence (biology) , peptide , monomer , oligonucleotide , microbiology and biotechnology , biochemistry , genetics , rna , polymer , gene , chemistry , organic chemistry
An artificial DNA duplex, each strand consisting of 45 monomers, is constructed from chemically synthesized deoxyriboöligonucleotides. The resulting bihelical polymer may code for a modified S-peptide of Ribonuclease A. This is the first synthetic duplex designed to code for a eukaryotic message.