Sequences spanning the EcoRI substrate site | Zendy

David E. Garfin | Zendy; Herbert W. Boyer | Zendy; Howard M. Goodman | Zendy

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Sequences spanning the EcoRI substrate site

Author(s) -

David E. Garfin,

Herbert W. Boyer,

Howard M. Goodman

Publication year - 1975

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/2.10.1851

Subject(s) - ecori , biology , restriction enzyme , dna , base pair , restriction site , restriction fragment , plasmid , nucleic acid sequence , restriction map , recognition sequence , genetics , microbiology and biotechnology , cleavage (geology) , paleontology , fracture (geology)

Substrate recognition by the EcoRI restriction endonuclease was investigated by analysis of the nucleotide sequences at the sites of enzymatic cleavage in various DNA molecules. 5'-end labeling and homochromatographic fingerprinting led to the determination of a 17-base-pair sequence spanning the EcoRI site of simian virus 40 DNA and a 15-base-pair sequence overlapping the EcoRI site of Col El plasmid DNA. Three other DNAs were similarly tested, although extended sequences were not determined in these cases. The EcoRI site was shown to be symmetric double-stranded equivalent of -N-G-A-A-T-T-C-N-.

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