Requirement of protein factors and ATP for the disassembly of the splicesome after mRNA splicing reaction | Zendy

Hitoshi Sawa | Zendy; Yoshiro Shimura | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Requirement of protein factors and ATP for the disassembly of the splicesome after mRNA splicing reaction

Author(s) -

Hitoshi Sawa,

Yoshiro Shimura

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.24.6819

Subject(s) - spliceosome , rna splicing , biology , ribonucleoprotein , protein splicing , messenger rna , precursor mrna , polypyrimidine tract , splicing factor , minor spliceosome , exonic splicing enhancer , microbiology and biotechnology , sr protein , rna , biochemistry , gene

Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research