Open AccessRequirement of protein factors and ATP for the disassembly of the splicesome after mRNA splicing reaction
Author(s) -
Hitoshi Sawa,
Yoichi Shimura
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.24.6819
Subject(s) - spliceosome , rna splicing , biology , ribonucleoprotein , protein splicing , messenger rna , precursor mrna , polypyrimidine tract , splicing factor , minor spliceosome , exonic splicing enhancer , microbiology and biotechnology , sr protein , rna , biochemistry , gene
Pre-mRNA splicing reaction occurs in a large ribonucleoprotein complex called the spliceosome. After the splicing reaction, the spliceosome is disassembled to release the splicing products including spliced mRNA. Here we show that protein factors in a HeLa nuclear extract or a DEAE-cellulose fraction as well as ATP are required to release the splicing products form the spliceosome in which the splicing reaction has already completed.