Open AccessTwo independent promoters as well as 5′untranslated regions regulate Ddrasexpression inDictyostellium
Author(s) -
Jean-François Louvion,
Jean-Charles Scholder,
Stéphane Pinaud,
Christophe Reymond
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.22.6133
Subject(s) - biology , promoter , dictyostelium , start codon , gene , untranslated region , reporter gene , gene expression , genetics , three prime untranslated region , microbiology and biotechnology , regulation of gene expression , rna , messenger rna
The Dd ras gene produces three transcripts during Dictyostelium development. The largest transcript (L-) can be induced by external addition of cAMP even in cells prevented from aggregating, whereas shorter transcripts (S1- and S2-) expression requires cell aggregate formation. We show the presence of two independent promoters for L- and S-transcripts by deletion analysis of Dd ras fragments fused to CAT reporter genes reintroduced in Dictyostelium. A direct repeat upstream of S-transcript start sites which seems involved in S-promoter function, modulates also L-RNA accumulation. Furthermore removal of sequences between this repeat and the AUG protein start codon reduces the level of L-transcripts in aggregates. This study allowed to uncover the intricate pattern of sequences participating in the regulation of Dd ras expression.