Different thermal energy requirement for open complex formation byEscherichia coliRNA polymerase at two related promoters | Zendy

Eileen A. Grimes | Zendy; Stephen Busby | Zendy; Stephen D. Minchin | Zendy

Open Access

Different thermal energy requirement for open complex formation byEscherichia coliRNA polymerase at two related promoters

Author(s) -

Eileen A. Grimes,

Stephen Busby,

Stephen D. Minchin

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.22.6113

Subject(s) - promoter , biology , rna polymerase , random hexamer , transcription (linguistics) , rna polymerase ii , consensus sequence , genetics , microbiology and biotechnology , rna , dna , gene , gene expression , base sequence , linguistics , philosophy

We have studied the effect of temperature on transcription initiation in vitro at two related promoters ga/Pcon and ga/P1, which have the same nucleotide sequence around the -10 region and transcription start site, but differ in upstream sequences. One of the promoters, ga/Pcon, carries the consensus -35 hexamer, 5'TTGACA 3', whilst ga/P1 contains a block of 'distortable' upstream sequences that allow promoter function in the absence of a -35 region consensus sequence. RNA polymerase can form complexes with both promoters at a range of temperatures. However, the thermal energy requirement for open complex formation differs: open complexes can form at ga/P1 at low temperatures, whereas ga/Pcon requires higher temperatures. The thermal energy requirement for transcription from preformed open complexes is the same for both promoters.

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