A short 5′ region of the long terminal repeat is required for regulation by hormone and heat shock ofDrospohilaretrotransposan 1731 | Zendy

Patrick Ziarczvk | Zendy; Martin BestBelpomme | Zendy

Open Access

A short 5′ region of the long terminal repeat is required for regulation by hormone and heat shock ofDrospohilaretrotransposan 1731

Author(s) -

Patrick Ziarczvk,

Martin BestBelpomme

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.20.5689

Subject(s) - biology , chloramphenicol acetyltransferase , long terminal repeat , retrotransposon , transcription (linguistics) , gene , microbiology and biotechnology , heat shock protein , genetics , psychological repression , promoter , gene expression , transposable element , linguistics , philosophy , genome

1731, a Drosophila retrotransposon was first described as having a transcription activity which was negatively regulated by 20-hydroxyecdysone (20-OH), the steroid molting hormone of insects. Using constructions expressing the bacterial chloramphenicol-acetyltransferase (CAT) gene under the control of the entire or deleted Long Terminal Repeats (LTRs) of 1731, we were able to show that a short (28 bp) sequence located in the U3 region of these LTRs was required for 1) the increase in promoter strength, 2) negative regulation by 20-OH and, 3) positive regulation by heat shock.

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