Open AccessA short 5′ region of the long terminal repeat is required for regulation by hormone and heat shock ofDrospohilaretrotransposan 1731
Author(s) -
P Ziarczyk,
Martin BestBelpomme
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.20.5689
Subject(s) - biology , chloramphenicol acetyltransferase , long terminal repeat , retrotransposon , transcription (linguistics) , gene , microbiology and biotechnology , heat shock protein , genetics , psychological repression , promoter , gene expression , transposable element , linguistics , philosophy , genome
1731, a Drosophila retrotransposon was first described as having a transcription activity which was negatively regulated by 20-hydroxyecdysone (20-OH), the steroid molting hormone of insects. Using constructions expressing the bacterial chloramphenicol-acetyltransferase (CAT) gene under the control of the entire or deleted Long Terminal Repeats (LTRs) of 1731, we were able to show that a short (28 bp) sequence located in the U3 region of these LTRs was required for 1) the increase in promoter strength, 2) negative regulation by 20-OH and, 3) positive regulation by heat shock.