Open AccessThe rewtrotramnsponcopiaregulatesDrosphilagen expresion both poatively and Negatively
Author(s) -
Linda A. Bryant,
Cathy Brierley,
Andrew J. Flavell,
John Sinclair
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.20.5533
Subject(s) - biology , transposable element , drosophila melanogaster , promoter , genetics , retrotransposon , dna transposable elements , gene , regulation of gene expression , regulatory sequence , gene expression , genome
The D. melanogaster transposable element copia is structurally similar to retroviral proviruses. We have asked whether copia encodes regulatory functions which have been observed in certain other proviruses. We have introduced reporter constructs based on the copia promoter and other Drosophila promoters into Drosophila cells and asked if copia has any affect on their expression. We find that, whilst copia negatively regulates expression from its own promoter, it also positively regulates expression from the larval serum protein 1 promoter. Analysis of RNA suggests that both regulatory functions occur by post-transcriptional mechanisms.