Open AccessAn erythroid specific enhancer upstream to the gene encodin the cell-type specific transcription factor GATA-1
Author(s) -
Silvia Nicolis,
C. Bertini,
Antonella Ronchi,
Stefania Crotta,
Luisa Lanfranco,
Emanuela Moroni,
Barbára Giglioni,
Sergio Ottolenghi
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.19.5285
Subject(s) - biology , enhancer , transcription factor , genetics , gene , gata transcription factor , transcription (linguistics) , gata2 , microbiology and biotechnology , gene expression , promoter , linguistics , philosophy
The transcription factor GATA-1 is expressed in a subset of hemopoietic cells, where it mediates the cell-type specific expression of several genes. We have cloned the mouse and human GATA-1 genes. A region upstream to the first exon, and highly conserved between mouse and man, acts as an erythroid specific enhancer in transient assays, if linked to the GATA-1 or to the SV40 promoter. The activity of the enhancer is almost completely dependent on the integrity of a dimeric GATA-1 binding site.