Open AccessStructural analysis of the actinophae ФC31 attachment site
Author(s) -
Helmut Rausch,
Martin Lehmann
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.19.5187
Subject(s) - biology , inverted repeat , prophage , genetics , recombination , direct repeat , dna , genome , binding site , base pair , a site , sequence (biology) , bacteriophage , base sequence , gene , escherichia coli
The lysogenisation of actinophage phi C31 in S. coelicolor J 1501 occurs by site-specific recombination. The DNA segments containing the attachment sites on the host chromosome, the phage genome and the two junctions created by the insertion of the prophage were cloned and the nucleotide sequences determined. The attachment sites (att) share an extremely short common sequence of three base pairs. Adjacent to the core sequences some direct- and inverted repeats were found as potential binding sites for proteins involved in site-specific recombination.