Open AccessAssociation of a change in chromatin structure with a tissue-specific switch in transcription start sites in the α2(I) collagen gene
Author(s) -
Konrad Beck,
Andreas Seekamp,
G. Roger Askew,
Mingxing Zhu,
Catherine Farrell,
S. Wang,
Lewis Lukens
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.18.4975
Subject(s) - biology , chromatin , transcription (linguistics) , genetics , gene , transcription factor , association (psychology) , microbiology and biotechnology , linguistics , philosophy , epistemology
Chick embryonic sternal chondrocytes do not synthesize alpha 2(I) collagen until they are shifted by treatment with 5-bromo-2'-deoxyuridine (BrdUrd) to a fibroblastic phenotype, yet they transcribe this gene as rapidly as BrdUrd-treated cells. To examine further this transcription, the DNase I hypersensitive sites were mapped in the 5' region of this gene in chondrocytes, BrdUrd-treated chondrocytes, fibroblasts and three types of non-transcribing cells. A DNase I hypersensitive site at -200 bp, previously shown to be associated with the active transcription of this gene in fibroblasts, is not present in chondrocyte chromatin. The chondrocyte alpha 2(I) gene contains, however, a novel major hypersensitive site in the DNA region corresponding to the fibroblast intron 2, near the chondrocyte-specific transcription initiation site of this gene. This novel hypersensitive site is associated with the use of this alternate start site by chondrocytes, since it is lost when BrdUrd treatment causes these chondrocytes to switch to the initiation of transcription at the fibroblast start site. The BrdUrd-treated chondrocytes contain the same alpha 2(I) hypersensitive sites as fibroblasts, except that fibroblasts have an additional, previously unreported, site at -1000 bp.