Open AccessPhysical map of chromosomal and plasmid DNA comprising the genome ofLeptospira interrogans
Author(s) -
Richard L. Zuerner
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.18.4857
Subject(s) - biology , leptospira interrogans , plasmid , restriction map , genome , restriction enzyme , genetics , chromosome , circular bacterial chromosome , physical mapping , genome size , dna , microbiology and biotechnology , gene , gene mapping , leptospira , serotype
The size and physical structure of the Leptospira interrogans genome was characterized using contour-clamped homogenous electric field (CHEF) gel electrophoresis. The L. interrogans genome is approximately 4750 kb in size and is composed of two molecular species of DNA: a 4400 kb chromosome; and a 350 kb plasmid, pLIN1. A physical map of the chromosome was constructed with the restriction enzymes NotI and SfiI. A physical map of pLIN1 was constructed with ApaI, NotI, Sse83871, SgrAI, and SmaI. Both the L. interrogans chromosome and pLIN1 are circular.