Open AccessA comparative study of the ribosomal RNA operons ofStreptomyces coelicolorA3(2) and sequence analysis ofrrnA
Author(s) -
Gilles P. van Wezel,
Erik Vijgenboom,
L. Bosch
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.16.4399
Subject(s) - ribosomal rna , biology , streptomyces coelicolor , operon , genetics , 16s ribosomal rna , 18s ribosomal rna , gene , 23s ribosomal rna , ribosomal dna , 5s ribosomal rna , sequence analysis , nucleic acid sequence , microbiology and biotechnology , rna , ribosome , phylogenetics , escherichia coli , mutant
S. coelicolor A3(2) contains six ribosomal RNA operons. Here we describe the cloning of rrnA, rrnC and rrnE, thereby completing the cloning of all operons. Southern hybridisation of genomic DNA with a heterologous probe from the E.coli rrnB 16S rRNA gene showed differences in hybridisation among the six rRNA operon-containing bands. The nucleotide sequence of the 16S rRNA gene and the upstream region of rrnA was determined and compared with the corresponding sequence of rrnD, showing that the 16S rRNA genes are 99% identical. Substantial differences were found, however, in the upstream regions corresponding to the P1 and P2 promoters of rrnD. Southern analysis showed that some of the other rRNA operons of S.coelicolor A3(2) also differed in this part of the upstream region.