Open AccessIdentification and sequence analysis of a methylase gene inPorphyromonas gingivalis
Author(s) -
Jeffrey A. Banas,
Joseph J. Ferretti,
Ann ProgulskeFox
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.15.4189
Subject(s) - biology , porphyromonas gingivalis , gene , methyltransferase , genetics , sequence analysis , homology (biology) , cytosine , open reading frame , peptide sequence , nucleic acid sequence , methylation , microbiology and biotechnology , dna methylation , dna , escherichia coli , bacteria , gene expression
A gene from the periodontal organism Porphyromonas gingivalis has been identified as encoding a DNA methylase. The gene, referred to as pgiIM, has been sequenced and found to contain a reading frame of 864 basepairs. The putative amino acid sequence of the encoded methylase was 288 amino acids, and shared 47% and 31% homology with the Streptococcus pneumoniae DpnII and E. coli Dam methylases, respectively. The activity and specificity of the pgi methylase (M.PgiI) was confirmed by cloning the gene into a dam- strain of E. coli (JM110) and performing a restriction analysis on the isolated DNA with enzymes whose activities depended upon the methylation state of the DNA. The data indicated that M.PgiI, like DpnII and Dam, methylated the adenine residue within the sequence 5'-GATC-3'.