Open AccessThe FIS protein binds and bends the origin of chromosomal DNA replication,oriC, ofEscherichia coli
Author(s) -
Hendrik Gille,
J. Barry Egan,
Angelika Roth,
Walter Messer
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.15.4167
Subject(s) - biology , seqa protein domain , escherichia coli , escherichia coli proteins , dna , dna replication , origin of replication , dna binding protein , genetics , microbiology and biotechnology , gene , transcription factor
The FIS protein (factor for inversion stimulation) is known to stimulate site-specific recombination processes, such as the inversion of the G segment of bacteriophage Mu, by binding to specific enhancer sequences. It has also been shown to activate transcription from rRNA promoters both in vitro and in vivo. We have identified a specific binding site for FIS in the center of the origin of chromosomal DNA replication, oriC. The DNA bends upon FIS binding. Occupation of the FIS site and binding of DnaA, the initiator protein, to its adjacent binding site (R3) are mutually exclusive. A fis mutant strain can not be efficiently transformed with plasmids which carry and replicate from oriC, suggesting that FIS is required for minichromosome replication.