Nucleotide sequence of aBacillus subtilisgene homologous to thegrpEgene ofE. colilocated immediately upstream of thednaKgene

' ^ I T * h C a t s h P c k f We thank B. Rak for computer work. This research project was ^ ^ upst^m oftfus gene, nucleodde sequencing a fr()m revealed an open reading frame coding for a protein of 187 ammo r r ° acids. This protein shows 50% homology to the grpE gene of £. coli (2). The nucleotide sequence determined by the dideoxy sequencing method (3) includes the complete sequence for the l Sussmann.M D and Sctlow.P (1987) Nud Acids Res 15, 3923 grpE homologous gene and part of the sequence of the dnaK gene. 2 Lipinska.B , Kmgj , Ang,D and Georgopoulos.C (1988)/^ AadsRes. Our sequence of the dnoK-gerK differs from that of the published 3 J ^ ^ 5 N(cklen s ^ Coulson A R (1977) P r o c Nall A ^ <* USA one (4) at four positions. Start and stop codons of the open reading 74 54^3 _ 54^ frame are underlined, a potential sigma-43 promoter is boxed. 4 Heame.c.M and Ellar.D J (1989) Nurf Acids Res 17, 8373