Open AccessThe cyanelle genome ofCyanophora paradoxa, unlike the chloroplast genome, codes for the ribosomal L3 protein
Author(s) -
Jean-Luc Evrard,
Clayton Johnson,
Ines Janssen,
Wolfgang Löffelhardt,
Jacques-Henry Weil,
Marcel Kuntz
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.5.1115
Subject(s) - biology , genome , genetics , ribosomal protein , chloroplast , ribosomal rna , computational biology , gene , ribosome , rna
We describe a 1132 bp sequence of the cyanelle genome of Cyanophora paradoxa containing the rpl3 gene. This gene, which is not chloroplast encoded in plants, is the first of a long cyanelle ribosomal operon whose organization resembles that of the S10 operon of E. coli. We have shown that the rpl3 gene is transcribed in cyanelles as a 7500 nucleotide precursor and that the 5'-end of the mRNA starts approximately 90 nucleotides upstream from the initiation codon. However, no typical procaryotic promoter could be found for this gene. We have detected, using anti E. coli L3 antibodies, the cyanelle L3 protein in cyanelle extracts and in E. coli cells transformed with the cyanelle rpl3 gene.