Open AccessDetermination of thecissequence involved in catabolite repression of theBacillus subtilis gntoperon; implication of a consensus sequence in catabolite repression in the genusBacillus
Author(s) -
Yoshiro Miwa,
Yuki Fujita
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.23.7049
Subject(s) - catabolite repression , biology , operon , bacillus subtilis , psychological repression , lac operon , fed batch culture , l arabinose operon , genetics , nucleic acid sequence , gene , promoter , consensus sequence , repressor , microbiology and biotechnology , biochemistry , peptide sequence , plasmid , bacteria , escherichia coli , gene expression , mutant , fermentation
The mechanism underlying catabolite repression in Bacillus species remains unsolved. The gluconate (gnt) operon of Bacillus subtilis is one of the catabolic operons which is under catabolite repression. To identify the cis sequence involved in catabolite repression of the gnt operon, we performed deletion analysis of a DNA fragment carrying the gnt promoter and the gntR gene, which had been cloned into the promoter probe vector, pWP19. Deletion of the region upstream of the gnt promoter did not affect catabolite repression. Further deletion analysis of the gnt promoter and gntR coding region was carried out after restoration of promoter activity through the insertion of internal constitutive promoters of the gnt operon before the gntR gene (P2 and P3). These deletions revealed that the cis sequence involved in catabolite repression of the gnt operon is located between nucleotide positions +137 and +148. This DNA segment contains a sequence, ATTGAAAG, which may be implicated as a consensus sequence involved in catabolite repression in the genus Bacillus.