The nucleotide sequence ofgreA, a suppressor gene that restores growth of anEscherichia coliRNA polymerase mutant at high temperature

We have recently reported the isolation of a suppressor RNA ACKNOWLEDGEMENT polymerase mutant of Escherichia coli that is susceptible to „ . , ,, __ , modification to a termination-resistant form by the phage lambda TM ' TM k ^ S U P P ° r t e d b y * ̂ ^° ** N gene product utilizing a mutant host factor, nusAl (1). The ° mutation, rpoB^, resulting from a serine to phenylalanine substitution at residue 522 of the beta subunit, also renders the REFERENCES cell temperature sensitive for growth (1). The proficiency of the , „ . . . . . „ . ,irw,> „ . . . , *^ 6 v / f j 1 Sparkowski J. and Das,A. (1990) Genetics, submitted. mutant RNA polymerase in general transcription activities and 2. Pabo and Sauer (1984) Ann. Rev. Biochem. 53, 293-321. gene expression at high temperature indicates that the F522 3. Helmann and Chamberlin (1988) Ann. Rev. Biochem. 57, 839-872. substitution has caused a loss of some specialized function of RNA Francklyn and Lee (1988) JBC 262, 4400-4407. polymerase, perhaps the interaction with a transcription factor . PTM " f^IL^j^l P^^^' 4 5 ~ 4 6 • . r i i ,,s rm_L • • ii • • 6. Angel el al. (1989) New BUA. 1, 3 5 4 3 . vital for cell growth (1). This hypothesis predicts that it may be 7 A v ^ a ^ (1985) ^J^Q J 4 457-463 possible to suppress the defect of the polymerase mutant by independent genetic events restoring the wild type interaction or bypassing the requirement for that interaction. Accordingly, we have tested whether the cell growth at high temperature can be restored by an E. coli gene cloned in a multi-copy plasmid. Here, £r^ia^5!i££iS?^^ we report the entire D N A sequence of a NcoI -BamHI fragment u

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