Open AccessNucleotide sequence encoding the major glycoprotein (GP2) of rat pancreatic secretory (zymogen) granule membranes
Author(s) -
Shin-lchi Fukuoka,
George A. Scheele
Publication year - 1990
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/18.19.5900
Subject(s) - biology , glycoprotein , microbiology and biotechnology
The major protein in the pancreatic secretory (zymogen) granule phase sequencing of purified protein. By use of the PCR product, membrane (1) is a glycoprotein (GP2) with molecular mass of two clones which contained the entire coding region of GP2 in ~ 80 KD in the rat (GP-80) and ~ 75 KD in the dog (GP-75). both orientations were purified from a rat pancreas cDNA library GP-75 was isolated from highly purified zymogen granule (ZG) constructed in Lambda-ZAP-II. The figure shows the complete membranes obtained from dog pancreas. Determination of amino nucleotide sequence including the open reading frame in these terminal and internal amino acid sequences allowed us to two clones. synthesize mixed oligonucleotide probes and employ these probes for replication of ds cDNA from mRNA using the polymerase REFERENCE Chain reaction (PCR). DNA sequence analysis of a 700 bp PCR ,. MacDonald.R.J. and Ronzio.R.A. (1972) Biodxem. Biophys. Res. Comm. product revealed the two peptide sequences identified by gas 49,377-382.